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The medium in the basolateral chamber was collected at different time points and fluorescence signals were recorded using a microplate reader (FLUOstar Omega, BMG Labtech, Ortenberg, Germany) at excitation/emission wavelengths of 480 nm/530 nm, respectively
This event was, however, not necessarily preceded by an oxidative burst and later on we demonstrated that GSH release was the result of an active process that the cells carried out through specific carriers in order to efficiently execute the apoptotic program ( necessary to induce the redox unbalance characteristic of the induction phase of the mitochondrial pathway but not sufficient per se to assure the execution phase of apoptosis
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