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All TLRs, with the exception of TLR3, primarily utilize the MyD88-dependent pathway, which leads to the activation of the key transcription factor NF-B and the subsequent upregulated expression of pro-inflammatory cytokines such as TNF-, IL-1, and IL-6

J Nanoparticle Res 15(7):1770

Gene expression profiling by quantitative reverse transcription-PCR Total RNA isolation from barley leaves was performed with GeneMATRIX Universal RNA Purification Kit (EURx, Gdask, Poland) according to the manufacturer's instructions (with additional treatment of RNase-free DNase I)