In this method, hot water (~78 C) was used to disrupt the yeast cells, releasing intracellular glutathione along with other water-soluble components, following which the suspension was rapidly cooled using ice and centrifuged at room temperature for 20 min
Third, aggregated peptides and immunogenicity
This novel modification type is present in various cellular compartments, and its extensive diversity in protein substrates is evidenced by its dual targeting of nuclear histones and cytoplasmic non-histones
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