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The prevalence of asthma has increased over recent decades, concomitant with the process of urbanization and industrialization (45)

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The slides were washed in water/PBS and then blocked using 5% goat serum in PBS for 1 h at room temperature prior to adding primary antibodies diluted in the blocking buffer

Additionally, coffee consumption could not be included in the analysis because the survey questions regarding coffee intake varied across the study years, preventing data pooling