Safety analysis of multi-week drug treatment in dogs One-year old canines (beagle dogs) with ulnar fractures were treated daily for 17 straight weeks subcutaneously with Ab 46 -D-Glu 20 doses of 0, 0.5, 1.6, or 5 nmol/kg/day
In brief, 0.2 L urine samples were analyzed by SDS-PAGE (10% gels) under reducing or non-reducing conditions and then transferred to polyvinylidene fluoride [PVDF (Immobilon -P Transfer Membrane, Merk Millipore Ltd., Burlington, MA, United States)] membranes by wet blotting (Mini Trans-Blot Electrophoretic Transfer Cell, BioRad Laboratories, Inc., Hercules, CA, United States)
Corticoids such as dexamethasone, methylprednisolone, and prednisolone were detected in post-administration study samples up to 7 h following a single therapeutic oral dose, whilst prednisone was not observed in a patients EB samples
Bioinformatics 22 , 26952696 (2006)