The existing methods for phenotypic selection of genetically modified mammalian cells are mainly based on three types of phenotypic markers: an exogenous drug resistance gene that permits screening for positive cells, a fluorescent protein, such as GFP, that allows isolating positive cells by FACS (fluorescence-activated cell sorting) ( Recently, we developed a gene transfer-positive cell sorting system using membrane anchoring Twin-Strep-Tag ( Glutathione-S transferase (GST) is a multifunction enzyme that catalyzes the conjugation of glutathione to electrophilic compounds ( Results Design the membrane anchoring glutathione S-transferase fusion maker We use membrane anchoring GST molecule as the affinity tag to allow the transfection-positive cells to be isolated using GSH beads (Figure 1)
Int J Biol Macromol 261(Pt 2):129813
doi: 10.1016/j.apsb.2021.07.028
Nanoscale 13 , 45054511 (2021) Liu, X., Kirlikovali, K.O., Chen, Z., Ma, K., Idrees, K.B., Cao, R., Zhang, X., Islamoglu, T., Liu, Y., Farha, O.K.: Small molecules, big effects: tuning adsorption and catalytic properties of metalorganic frameworks