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Vitamin and mineral supplementation for preventing dementia or delaying cognitive decline in people with mild cognitive impairment

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The diagnosis of vitamin B12 deficiency typically relies on low total B12 levels and elevated concentrations of homocysteine (Hcy), as well as levels of transcobalamin-bound B12 (holoTC) and methylmalonic acid (MMA), which serve as functional indicators of cobalamin insufficiency (Carmel and Sarrai, 2006)

80 of each plasma sample was transferred to previously prepared tubes containing 240 \xL ice-cold acetonitrile and vortexed vigorously