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Common amounts: 1ml for high concentration (smaller injections) 2ml for medium concentration (balanced) 3ml for low concentration (easier to measure small doses) Step 3: CRITICAL TECHNIQUE - Aim needle at the inside wall of the vial, NOT directly at the powder

One possible explanation for this difference is that nonsynaptic mechanisms of [Ca 2+ ] i rise, VGCCs, and release from internal stores were activated by the AP-only protocol sufficiently strong to produce calcium levels necessary for triggering plasticity all over the dendritic tree

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