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The following reduction of GSH cell concentration and increase of ROS levels also contributes to cell death 88

Intracellular ROS level using H2DCF-DA, and Nrf2 activation by ELISA were measured

RNA was quantified by one-step real-time quantitative reverse transcription PCR (qRT-PCR) using the HiScript II One Step qRT-PCR SYBR Green Kit (#Q221-01, Vazyme, Nanjing, China)