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The reaction was performed in 150 l of 50 mM Tris buffer (pH 8.0) containing 20 M Cp1D or Cp2D, 15 mM ATP, 5 mM N -succinyl amino acid substrate, 200 mM hydroxylamine and 10 mM MgCl 2

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Besides managing the underlying issue, the following treatments can be undertaken after doctors prescription/done by an expert for lightening of lip colour