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The supernatant was discarded, and the RNA pellet was washed three times with 1 mL of ice-cold 70% ethanol (Roth)

However, in specific scenarios, such as those involving participation in multiple events within short intervals, athletes might prioritize rapid recovery or reduced soreness over long-term adaptation

Payers reviewing D62 claims expect estimated blood loss documented in the operative or emergency note, vital sign changes indicating hypovolemia such as tachycardia and hypotension, and a treatment plan involving transfusion or fluid resuscitation