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To me, the fear of conflict was pretty much gone because I was just engaging in conflict all over the country

Azuma, Y

Prior to any procedure, animals are anesthetized using a combination of xylazine (10 mg/kg), ketamine (40 mg/kg) and acepromazine (0.75 mg/kg) for up to an hour via the intramuscular route

The weight-drop, midline fluid percussion, and controlled cortical impact models produce focal contusions and, depending on injury severity, may be associated with subarachnoid and intraparenchymal hemorrhages, disruption of the blood-brain barrier (BBB), cortical and subcortical cell loss, and diffuse axonal injury