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Tissues were fixed with 10% buffered formalin, and paraffin-embedded sections were stained with hematoxylin and eosin (H&E)

These studies were deemed insufficient to warrant changes because they: (1) relied on mothers self-reported APAP usage, (2) lacked precise quantification of APAP dosage, and (3) frequently utilized questionnaire-based methodologies

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(8) Furthermore, FOXO4's role in the IIS pathway suggests it might modulate the actions of diet and environmental factors on cell aging, acting as a mediator between external factors and cellular longevity pathways