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Peptide Reconstitution Protocol Standard laboratory reconstitution protocol for lyophilised peptides using bacteriostatic water: Preparation Allow the peptide vial and bacteriostatic water vial to reach room temperature (reduces condensation) Wipe the rubber stoppers of both vials with an alcohol swab (70% isopropanol) Allow the alcohol to evaporate completely before piercing the stopper Reconstitution Steps Draw the required volume of bacteriostatic water into a sterile syringe (typically 12 mL for 10 mg peptides) Inject the water slowly down the inside wall of the peptide vial (not directly onto the powder) Allow the water to gently flow over the lyophilised powder without forceful spraying Do not shake the vial

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N., Oh, J., Branch, K