Proteins were separated on Bis-Tris SDSPAGE, transferred onto nitrocellulose membrane and probed with anti-p-eIF2 (Cell Signaling Technology, 3597), anti-eIF2 (Cell Signaling Technology, 2103) anti-p-ACC (Cell Signaling Technology, 3661), anti-ACC (Cell Signaling Technology, 3662), anti--tubulin (Proteintech, 10068-1-AP) and anti--ActinPeroxidase antibody (Sigma-Aldrich, A3854)
For hyperpigmentation, uneven skintone, dark spots on face, dark spots on body, dark underarms, dark knees & elbows, dark inner thighs, kojic acid soap, brightening kojic acid lotion, skin lightening vs skin brightening, dark neck, cysteamine, glycolic acid, arbutin, azelaic acid, dark circles, dark knuckles, melasma
Her goal is to make the experience feel like a partnership, helping patients navigate their options with confidence and clarity
The absorbance value at 540 nm was measured using a microplate reader