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The wtVP3 and wtVP0 expression cassettes were released from HC-wtVP0 and HC-wtVP3 by BamH I and Bgl II digestion, respectively, and then sequentially inserted into HC-wtVP1 from the Bgl II site, yielding plasmid HC-wtVP 031 that contained all three (wtVP0, wtVP3, wtVP1) expression cassettes