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Determination lipid peroxidation activity Lipid peroxidation activity was measured in the form of malondialdehyde using thiobarbituric acid based on Stocks and Dormandy method with some modifications [27]

His-38 and His-50 are located within polar interaction distance of the glycyl carboxylate moiety of GSH [80, 84, 85]

Based on these observations, it is plausible to propose that in ovarian granulosa cells and oocytes, MAMs may contribute to cellular functional outcomes, such as cryotolerance and steroidogenic efficiency, by regulating phospholipid exchange and membrane properties, including the PS/PE balance