This molecular event triggers formation of multi-protein signalling complexes containing paxillin, talin, and vinculin, detectable through co-immunoprecipitation assays and proximity ligation microscopy techniques
Quantification of CLEC7A by flow cytometry Cells were adjusted to a concentration of 5 10 5 cells/ml in PBS containing 0.5% BSA and incubated with the PE-conjugated CD369 (Clec7a, Dectin-1) monoclonal antibody (Thermo Fisher Scientific, 129856-42, RRID: AB_2572749) and the corresponding IgG2a-PE isotype control (Thermo Fisher Scientific, 12-4724-82, RRID: AB_470064) (dilution 1:20) for 20 min at 4 C
J., Kliewer, S
When used in topical formulations, the most commonly reported issues are localised and mild, and may include: Skin irritation or redness at the site of application Contact dermatitis in individuals with sensitive skin or known peptide sensitivities Scalp itching or tingling , which may be transient The following precautions are advised: Avoid application to broken, inflamed, or irritated skin , and keep the product away from the eyes and mucous membranes
