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Mutant generation The N-terminal 6xHis version of plasmid pSP19T7LTMGST1, containing wild-type rat MGST1 was used as the template for site-directed mutagenesis 47

Trimethylamine N-Oxide: A Link among Diet, Gut Microbiota, Gene Regulation of Liver and Intestine Cholesterol Homeostasis and HDL Function

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We then pre-incubated early ring-stage artemisinin resistant and sensitive parasites with BSO for 1 h prior to pulse treatment with 700 nM of DHA for 6 h and measured survival, analogous to the standard in vitro Ring-stage Survival Assay (RSA) for artemisinin resistance