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The DIO mice were randomized into three treatment groups balanced by baseline body weight and body composition (fat and lean mass) measures

8 weeks (11 vials): 33 mL 4 10 mL bottles 12 weeks (17 vials): 51 mL 6 10 mL bottles 16 weeks (22 vials): 66 mL 7 10 mL bottles Alcohol Swabs: One for the vial stopper + one for the injection site each day

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