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The MB was then added at 7-serial dilution concentrations (0.14 to 100 M) and incubated for 24, 48, or 72 h (37 C and 5% CO 2 )

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Lunetta KL, D'Agostino RB, Karasik D, Benjamin EJ, Guo CY, Govindaraju R, Kiel DP, Kelly-Hayes M, Massaro JM, Pencina MJ, Seshadri S, Murabito JM: Genetic correlates of longevity and selected age-related phenotypes: a genome-wide association study in the Framingham Study