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Western blotting Western blotting was performed as previously described 6

For most people, sustainable fat loss depends more on: Calorie balance Protein intake Physical activity Sleep quality Long-term consistency Supplements may provide additional support, but they do not replace healthy habits

C-terminal modification of macrocycles For fluorescein- or biotin-labelling of aMD5, aMD5-Lys(Mmt)-NH-resin was synthesized by Fmoc SPPS and the Mmt group was then deprotected using 98% dichloromethane, 1% TFA and 1% triisopropylsilane

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