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The sections (5 m) were stained with 5 M DHE at 25 C in the dark for 1 h according to the manufacturers instructions

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Despite these few differences, both the explanted heart and the mouse model had fibrosis, hypertrophy, remodeling of Ca 2+ cycling (decrease in SERCa2 levels accompanied by a compensatory increase in NCX levels), metabolic remodeling (inhibition of Akt activity coupled with upregulation of AMPK activity), and oxidative stress (increased MDA levels and low GSH/GSSG ratio)

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