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RNA content of the isolated MVs The RNA content of the MVs was extracted using the RNX Plus kit (SinaClon, Tehran, Iran)

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Nevertheless, these enzymes can also be released into extracellular compartments, including serum, under physiological or pathological conditions 46

Thus, the discovery positions GGCT as a promising therapeutic target for disrupting metabolic plasticity through its dual roles in Gln shunting and Glc compensation