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(DMEM/F12, Gibco, Gaithersburg, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Gibco, Gaithersburg, USA) and 1% penicillin/streptomycin (Invitrogen, Life Technologies, USA) in a humidified incubator with 5% CO 2 at 37 C

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