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It highlights the distinction between adaptive circadian remodeling during normal aging and pathological circadian disruption linked to disease, providing a conceptual framework for understanding age-dependent alterations in sleepwake regulation

For differentiation, 12 10 5 PBMCs were seeded in 1 ml complete medium containing 1 penicillinstreptomycin and 2050 ng ml 1 GM-CSF (PeproTech) per well in 24-well plates for 46 days, with medium changed every other day

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11 Gumina S, Passaretti D, Gurz MD, Candela V