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Antagonists Antagonistic analogs of LH-RH In 1971, we also proposed that the replacement or deletion of some amino acids in the decapeptide sequence of LH-RH might result in analogs possessing structure requirement features for effective binding, but devoid of features necessary for a functional effect 16

In brief, cell pellets (4 to 6 10 6 cells) collected in phosphate-buffered saline (PBS) containing dibutylhydroxytoluene (BHT, 100 M) and diethylenetriamine pentaacetate (100 M) were washed and centrifuged

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Nonetheless, certain variations in the drugs effects can be discerned