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doi: 10.1111/j.1440-1827.2008.02250.x

Formation and dispersal of acetylcholine receptor clusters in muscle cells Trends Neurosci 1986

For gene expression analysis, 1 g of total RNA for each sample was retrotranscribed into complementary DNA (cDNA) with the iScript TM cDNA Synthesis Kit (BIORAD, Hercules, CA, USA), following the manufacturer's instructions, using the GeneAmp PCR System 9700 (Perkin Elmer, Waltham, MA, USA)