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The homogenates were centrifuged at 20,400 g for 20 min at 4 C, and the resulting supernatants were appropriately diluted and used in the assays, which were conducted according to the manufacturers instructions

Researchers have proposed several mechanisms, including direct effects on gene transcription factors and indirect influences through cellular signaling cascades that regulate telomerase activity

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In a cross-sectional study based on National Health and Nutrition Examination Surveys, higher visceral adiposity index is independently related to ED risk [141], The visceral adiposity index can clearly reveal the influence of waist circumference, body mass index, triglyceride, etc., and is considered more practical in assessing the impact of obesity on ED patients [142]