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Several plausible mechanisms have been proposed to understand the role of OS in causing BPH

Image analysis software was used to read the band intensity of the target proteins against the control sample after normalization by -actin ( 2.7 Histopathological analysis Hepatic injury can be detected by hematoxylin and eosin (H&E) staining of liver tissues ( The formalin-fixed liver specimens were inserted in liquified paraffin wax and cut into 5-m thick sections utilizing a Leica microtome (Leica RM 2155, England)

Dual luciferase assay Fragments of either CBS-3UTR-WT or CBS-3UTR-Mut (where m 6 A was changed to T) were cloned into pmirGLO vectors to generate CBS-3UTR-WT and CBS-3UTR-Mut plasmids (Table S5)

Therefore, a definite interpretation of more decreasing effects of NAC on E2 levels than metformin needs more studies