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Protein preparation involved the removal of co-crystallized ligands, ions, and water molecules, followed by the addition of polar hydrogens and Kollman charges using AutoDock Tools (ADT)

Chambers RC, Leoni P, Kaminski N, Laurent GJ, Heller RA: Global expression profiling of fibroblast responses to transforming growth factor-beta1 reveals the induction of inhibitor of differentiation-1 and provides evidence of smooth muscle cell phenotypic switching