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Enzymatic Reaction Conditions The substrate solution used for the NsPCS reaction was prepared as follows: 28.7 mg of tris (2-carboxyethyl) phosphine hydrochloride (TCEP) (Tokyo Chemical Industry) and 3.0733 g of GSH (Nacalai Tesque) were weighed in a 100 mL volumetric flask, 20 mL of 1000 mM potassium phosphate buffer (pH 8.0) was added, and the solution was gently shaken
Meanwhile, this strain can induce IL-10 secretion by secreting polysaccharide A (PSA), synergistically promote Treg proliferation, reduce the levels of pro-inflammatory cytokines such as TNF- and IL-6, and thus inhibit the systemic inflammatory response in RA
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