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In addition to the copper-induced accumulation of lipoylated mitochondrial DLAT enzymes, there is also a loss of ironsulfur (Fe-S) cluster proteins, leading to proteotoxic stress [21,39,40,41]

Samples were diluted with 50 mM Tris-HCl to bring urea concentration down to below 2 M to prevent trypsin inhibition

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HOW TO INCREASE GLUTATHIONE IN YOUR BODY Natural glutathione production declines with age (when you need it most), but you can increase glutathione levels through lifestyle choices