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When you stack MOTS-c 5-Amino-1MQ, the most effective approach is staggered introduction: establish baseline metabolic parameters (fasting glucose, insulin sensitivity, body composition), introduce MOTS-c alone for 1014 days while monitoring those parameters, then add 5-Amino-1MQ and observe whether the combination produces additive or synergistic effects

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Together, our observations that a small molecule NNMT inhibitor can increase NAD + and SAM concentrations in differentiated adipocytes and reduce adiposity in DIO mice, suggest that treatment with an NNMT inhibitor effectively reverses obesity via modulation of the NAD + salvage and SAM-mediated (e.g., polyamine flux) pathways to regulate energy expenditure in the adipose tissue

Summers, Anne Thorburn, Esra Ogru, Robert Gianello, Woei-Jia Jiang, Frank M