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For IHC staining, primary antibodies (GPX4 (ab125066), SLC7A11 (ab307601), IL-6 (21,8651-AP), TNF- (26,4051-AP), COX2 (27,3081-AP), and MMP13 (18,1651-AP)) were applied at a dilution of 1:200 and incubated overnight at 4 C, followed by incubation with an HRP-conjugated secondary antibody and DAB color development

However, the clinical significance of this is unclear (144)

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