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After reaching 70% confluence, fibroblasts were cultured for 24 h in 1% FBS DMEM and then incubated with TGF1 10 ng/ml (Peprotec, Canada), GHK 10 nM (Sigma, Canada) or growth media control for a further 48 h

7.2 Standardization and quality control Given that the bioactive components of Scrophulariae Radix vary with plant origin, cultivation, and processing, standardized extraction methods and strict quality control are essential to ensure its stability, consistency, and therapeutic reliability for modern medical use

It is especially useful where oral intake is insufficient or absorption is compromised, as noted in the product literature