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the guide cannula measured 2.5 cm in overall length and was prepared with a heat bulge placed 2.5 mm from its beveled tip, thus acting as a stop to control the depth of penetration at 2.5mm, Once in position, the cannula was secured to the skull with two stainless-steel screws and dental cement

By increasing Ca 2+ influx and consequently insulin granule exocytosis, both pathways work together to promote insulin secretion

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