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Several clinical circumstances involve the oxidation of tetrahydrobiopterin (BH4) and mitochondrial electron transport, as well as the inactivation of nicotinamide-adenine dinucleotide phosphate (NADPH) oxidase, xanthine oxidase (XO), cyclooxygenase, and uncoupled endothelial nitric oxide synthase (eNOS) ( 5.3

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The strategy adopted has used the Strep -tagged GSTs as protein hooks, which when either expressed in planta transiently ( Nicotiana benthamiana ), or stably (Arabidopsis) bind endogenous ligands in the host which can then be recovered following affinity capture of the fusion protein (Figure 1)

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