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Samples were washed x3 with cold argon-purged 50 mM HEPES buffer (pH 7.5), resuspended in 500 L washing buffer and exposed first to 1 mM TCEP, and after 3 min, 5 mM IAM was added and incubated for a further 20 min (Procedure A) or IAM-TPP (Procedure B) for 30 min at RT protected from light