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2 Materials and methods 2.1 Reagents Dextran sulfate sodium salt (DSS, MW 36,00050,000 Da, batch number 160110) was purchased from MP Biomedicals

[15] [16] NICE guidance (CG189: Obesity identification, assessment and management, and PH53: Weight management lifestyle services) supports the use of total diet replacement programmes only within structured clinical settings with appropriate monitoring and multidisciplinary support