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(5) Subjects who have donated blood or lost more than 400 mL of blood within the past 3 months, or who plan to donate blood during the study period

Joint pain, stiffness, swelling, and reduced mobility often affect sleep, exercise, work, and overall quality of life

Eating smaller, more frequent meals rather than large portions reduces gastric distress

Additionally, chemoaffinity protocols such as immobilized metal affinity chromatography (IMAC) or titanium dioxide (TiO 2 ), although widely used in phosphoproteomics, lack site selectivity for phosphorylation at serine (pS), threonine (pT) or tyrosine (pY) 8,9 and exhibit a sequence bias in favor of peptides rich in aspartic (D) and glutamic (E) acid