Models predict that VEA decomposes into several highly reactive intermediates when heated to temperatures reached during dry hit vaping including 4-acetoxy-2,3,5-trimethyl-6-methylene-2,4-cyclohexadienone (ATMMC), duroquinone (DQ), duroquinone methide (DQM), and ketene, which is known to be acutely toxic
Cathepsin B activity was then assayed in final buffer conditions of 0.04 ng l 1 cathepsin B, 40 M Z -Phe-Arg-AMC (Sigma), 40 mM citrate phosphate (pH 5.5), 1 mM EDTA, 100 mM NaCl, 5 mM DTT and 0.01% Brij at 37 C
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182,183 Thus, parents of autistic children are likely to believe that vaccines are responsible for the induction of autism not because of a peer-reviewed article but rather because of their observations and the observations of individuals within their trusted social networks
