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Briefly, to verify the targeted binding between miR-29b-3p and the GGCT gene, a 2000-bp DNA fragment (derived from the transcription start site of the GGCT gene) was inserted into the pmir-GLO-Basic vector to construct the wild-type pmir-GLO-GGCT-3 UTR reporter plasmid (wt) and the mutant pmir-GLO-GGCT-3 UTR reporter plasmid (mut)

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Senolytic Research: Some bioactive peptides are being investigated for their ability to selectively induce apoptosis in senescent (zombie) cells, a process that may reduce the chronic inflammation characteristic of aging

doi: 10.1046/j.1365-2826.1999.00351.x 5 BennettP