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A protocol designed around smaller, more frequent measurements typically calls for a different concentration than one built around larger, less frequent draws and since concentration is simply peptide mass divided by liquid volume, changing either side of that relationship changes the correct water volume

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Investigators are particularly interested in determining the mechanisms by which effects observed in murine models relate to broader mammalian metabolic regulation, and whether different experimental conditions (young vs aged models, healthy vs metabolically compromised states) show differential responses to NNMT inhibition

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