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5 Reward-associated mechanisms explain why individuals search for initially positive stimuli again and again and why this can ultimately lead to compulsive behaviors and addictions that pose a threat to mental health

2.4 SA--Gal stain SA--Gal staining of cells and tissues was performed using a senescence-associated -galactosidase staining kit (C0602, Beyotime Biotechnology, Shanghai, China), with all procedures strictly following the manufacturers instructions

This is a meaningful evidence gap

However, our internal experience suggests that for the most sensitive research applications, potency can begin a measurable decline even before that point