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Methods T1 MRI data were acquired from 133 prospectively enrolled subjects for this observational study (80 cLBP, 53 controls

To induce barrier damage cells were incubated with proinflammatory cytokines (CK) tumor necrosis factor- (TNF-) and interleukin-1 (IL1-) both at 10 ng/ml concentration

Additionally, endotoxin levels are verified using the LAL test (Limulus Amebocyte Lysate) to exclude contamination

We provide evidence that even in this cohort representing healthy aging, several markers related to oxidative damage differ significantly from that of young adults, suggesting a pro-oxidative systemic environment