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In one acromegalic patient presenting with nephrotic range proteinuria and focal segmental glomerulosclerosis on kidney biopsy, proteinuria quickly normalized after tumor removal but returned 4 months later, but responded to prednisolone treatment [94]

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Luciferase report gene assay Cellular cAMP determinations were based on an indirect method using a reporter plasmid containing the firefly luciferase gene under the control of multiple cAMP-responsive elements (CREs) and pRL-TK as a control vector (Promega Corp., Madison, WI), which has previously been used for determining D 1 R, D 2 R, and D 3 R signaling [31, 32]