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Weiss GA, Hennet T (2017) Mechanisms and consequences of intestinal dysbiosis

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Mammalian cell culture medium was centrifuged twice at 12,000 g for 20 min at 4 C, later the supernatant pH was adjusted to 8.0 with equilibration buffer (50 mM sodium phosphate, 300 mM NaCl and 20 mM imidazole, pH 8.0)

761 The previously noted strong evolutionary similarity of bacterial ZigA/ZagA to the mycobacterial Mrf (MPY recruitment factor) deserves comment