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LH has a biological half-life of 20 minutes

Cell-free supernatants were collected and stored at -20C for the future assays and granulomas were harvested by adding ice-cold sterile 1x PBS to wells, followed by gentle scraping to achieve maximum recovery of granuloma lysates and stored at -20C

Less common reports include fatigue 1224 hours after dosing, injection-site redness, and brief flushing

ProHealth's FAQ notes that many users prefer taking it in the morning for routine consistency, and that some studies suggest absorption may be enhanced when taken with food - though the brand describes the arginate salt as stable in the stomach environment regardless