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The mechanism by which probiotics exert their positive effects is still not known but it is though that they might influence neuroactive metabolites like gamma-aminobutyric acid and serotonin (Israelyan and Margolis, 2018)

Peak 1 was tentatively identified as p-hydroxybenzoic acid, displaying a deprotonated molecular ion [M H] at m/z 137.0251

It also acts as an antioxidant, preventing CVD (250, 251), and has diuretic and antiproliferative properties

To determine albumin secretion, supernatant from 24 h was collected and the amount of albumin was determined using a human-specific albumin ELISA kit (Assay Pro) following the manufacturers instructions on an ELISA plate reader (Tecan Spark 20M)